Figure 2.

Correlation between single-cell RNA-seq and single-cell multiplexed qPCR for each sample preparation method. Correlation coefficients were computed from log₂- transformed values. A linear regression line (color) and the y = x line (black, dotted) are also shown in each panel. Shading represents the 95% confidence interval for each regression line. For RNA-seq data, FPKM values for each of gene of interest were normalized to the median FPKM for each cell and log₂ transformed. For qPCR data, threshold cycle (Ct) values for each gene of interest were normalized to the median Ct value for each cell (ΔCt), which also represents a fold change over the median expression. ΔCt values are already in log₂ space and were directly plotted. Error bars, standard error (n values as in Fig. 1a).