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. 2014 Mar 12;2(3):354–358. doi: 10.3892/br.2014.250

Figure 3.

Figure 3

(A) Apoptosis induction with radiation in siRelB-RM-1, siVector-RM-1 and non-trans-RM-1 cells. The apoptosis rate was measured with Annexin V/PI flow cytometry in the various RM-1 cells that were treated with 6 Gy radiation, along with the untreated control RM-1 cells. Radiation treatment induced apoptosis in all the treated cells compared with the control cells. (B) Irradiation activates Bcl-xl in RM-1 cells. The cells were pretreated with siRelB prior to irradiation. The RM-1 cells were subjected to qPCR assay. Total RNA isolated from irradiated cells and untreated control RM-1 cells. (C) Bcl-xl mRNA levels were measured by qPCR assay normalized by the level of β-actin. Significant differences were observed, as indicated, when compared with the untreated groups (*P<0.05 and**P<0.01 vs. control). si, small interfering; control-RM-1, non-irradiated RM-1 cells; non-trans-RM-1, non-transfected RM-1 cells; siVector-RM-1, RM-1 cells transfected with empty vector; siRelB-RM-1, pLentilox-sh-RelB-transfected RM-1 cells; qPCR, quantitative polymerase chain reaction.