Figure 3.

Histology and morphology of human tumor-derived pancreatic stellate cells. (A) Representative phase-contrast image of human tumor-derived pancreatic stellate cells in monolayer after 72 h in culture. EUS-FNAs were placed in standard cell culture conditions, resulting in the outgrowth of myofibroblast-like cells. These stellate cells are characterized by a flattened angular appearance, the presence of cytoplasmic lipid droplets, long cytoplasmic processes giving them a typical “stellate” appearance, and cytoplasmic expansion and contraction behavior. (B) Representative hematoxylin/eosin (H&E) stained section and phase-contrast bright field images from time-lapse movies of cell cultures from patients with locally advanced, unresectable pancreatic adenocarcinoma before treatment (pre) and at 6 months following treatment with gemcitabine (G) and stereotactic radiosurgery (SRS) (post). White arrowheads point to cytoplasmic lipid droplets; black arrows point to lipid-droplet containing pancreatic stellate cells; white arrows point to other non-stellate cells in culture; black arrowheads point to pancreatic ductal adenocarcinoma cells. Scale bar: 50 μm.