Figure 3. Structures of repressed and hyperactive BAP-ClpP mutants.
(A) Side view (left) and 35 Å section (right) of the BAP-E432A map with the ClpB hexamer fit. (B) Fitting of the BAP-E432A AAA+ rings. (C) Side view (left) and 35 Å section of the fitted BAP-Y503D structure. MD is only partially in density and is more tilted than in the wild type and the BAP-E432A repressed mutant. The ND is smeared out as in all BAP forms due to disorder, but has a more vertical orientation in the hyperactive state. (D) Fitting of the BAP-Y503D AAA+ rings. The motif 2 region protrudes from the density.
Figure 3—figure supplement 1. Test of EM map refinements after interchange of starting models.
