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. 2014 May 5;13:102. doi: 10.1186/1476-4598-13-102

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Copyright © 2014 Hoshino et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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HOXB9 expression in colorectal cancer. (a) Waterfall plot of relative mRNA expression levels of HOXB9 in patients at the cancer site compared with those in normal mucosa (n = 69), determined using qRT-PCR in triplicate. (b) Immunohistochemical staining of HOXB9 in resected colorectal cancer specimens (T, tumor; N, normal mucosa). (c) Relative mRNA expression of HOXB9 in patients according to pathological differentiation (Well/ mod, well or moderately differentiated adenocarcinoma; Por, poorly differentiated adenocarcinoma). (d) Kaplan-Meier curves of colorectal cancer patients (n = 93), according to HOXB mRNA expression (Left panel, disease free survival; right panel, overall survival). (e) mRNA expression of HOXB9 determined by RT-PCR. (f) Immunocytochemistry of HOXB9-overexpressing cell lines. (g and h) mRNA expression of angiogenic factors (g) and TGF beta family (h) in HOXB9-overexpressing cell lines using RT-qPCR in triplicate. Error bars are SDs. P values by the log rank test. Scale bars, 100 μm.