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. 2014 May 5;13:102. doi: 10.1186/1476-4598-13-102

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Copyright © 2014 Hoshino et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Impact of HOXB9 expression on tumor xenografts. (a, b) Subcutaneous xenograft model of HT 29-T cells was established in Balb/c nu/nu mice (n = 8, each). Tumor burden (a) and tumor weight (b) were evaluated. (c) Immunohistochemical staining of paraffin-embedded xenografts obtained from HT29-T cancer cells. Samples were stained in anti-HOXB9 or anti-CD31. (d) Microvessel density of xenograft tumor was evaluated and measured in immunohistochemical staining by ant-CD31 antibody in xenografts (n = 8, each). Error bars are SDs. ☆ p < 0.05 and † p < 0.005 by U-test. Scale bars, 200 μm.