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. 2014 May 5;13:102. doi: 10.1186/1476-4598-13-102

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Copyright © 2014 Hoshino et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Impact of cytokine release from HOXB9 overexpressed cells on angiogenesis and cell proliferation. (a) Cancer cells (3000 cells/well) were seeded on micropore membrane over 24-wells plate and incubated for 24 h before tetracycline administration and/or co-culture with HUVECs and HDFs. After 48 hours incubation with tetracycline and/or co-culture system, the number of cancer cells were counted (n = 8, each). (b) Total capillary length of co-cultured HUVECs were evaluated in immunohistochemical staining of CD31 after 48 hours incubation with HOXB9 induced cell lines (n = 8, each). Error bars are SDs. ☆p < 0.05 by the U-test.