Table 1. Predicted polymorphic miRNA target sites of DISC1.

SNP	Positiona	MAF CEU	Octamer (‘seed')b	Effect	SNP allele	Targeting miRNA (stem loop)	Stem loop location	Mature miRNA
rs11122396	230241891	0.042	caAGCC[A]Taactc	C	A*	miR-135a-1	3p21	hsa-miR-135a-5p
				C		miR-135a-2	12q23.1
				C		miR-135b	1q32	hsa-miR-135b-5p
					G (ancestral)	None
rs980989	230242818	0.217	tttACA[T]TCAtat	C	T	miR-409	13q32.3	hsa-miR-409-3p
					G* (ancestral)	None
rs9308481	230242929	0.225	taaAAT[G]TGAagt	N	G* (ancestral)	miR-323	14q32	hsa-miR-323-3p
			aaaAT[G]TGAAgt	N		miR-23a	19p13	hsa-miR-23a-3p
				N		miR-23b	9q22.3	hsa-miR-23b-3p
			AAT[G]TGAA	N		miR-130a	11q12	hsa-miR-130a-5p
					A	None
rs11803088	230243392	0.069	cttTTA[C]TTTtaa	N	C* (ancestral)	miR-559	2p21	hsa-miR-559
			cttttA[T]TTTTAa	D	T	miR-548c	12q14.2	hsa-miR-548c-3p

Predictions from Patrocles and PolymiRTS are shown, with miRNAs and SNPs predicted by both programs underlined. The SNP alleles of the wild-type DISC1 Lv construct are indicated with an asterisk (*). The effect of the SNP on the target site is denoted as follows: C=derived allele creates a novel miRNA binding site, N=derived allele disrupts a non-conservative miRNA binding site, D=disrupts a conservative miRNA binding site. MAF=minor allele frequency; CEU=HapMap CEPH population.

^aRefSeq build 36.3.

^bGenomic sequence 5′ to 3′.