Skip to main content
. 2014 Feb 14;139(1):142–161. doi: 10.1093/toxsci/kfu028

FIG. 1.

FIG. 1.

Neuronal differentiation of hESCs. (A) Schematic representation of the differentiation protocol and Pb exposure paradigms. (B) WA09 hESC cell colony on mouse embryonic fibroblast (MEF) feeder layer. hESCs are immunoreactive for the pluripotency markers Oct4 (C), Nanog (D), and Lin28 (E). (F) hESC-derived embryoid bodies (EBs) at culture day 6. (G) Formation of neural rosettes (arrows) 6 days following plating of EBs on fibronectin-coated plates. (H) hESC-derived NPCs display immunofluorescence labeling for the NPC markers Nestin (red) and Musashi1 (Msi1) (green). (I) At day 47 of the differentiation protocol, hESC-derived neurons express the neuronal-specific marker βIII-tubulin (TUJ1 antibody). (J) hESC-derived neurons also express the mature neuronal marker MAP2 at 31 days of the differentiation protocol. Nuclei are stained with DAPI. Bars: 100 μm (B)–(G); 50 μm (H)–(J).