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. 2014 Feb 14;139(1):142–161. doi: 10.1093/toxsci/kfu028

FIG. 4.

FIG. 4.

Chronic exposure to Pb alters the expression levels of the neural markers MSI1 and PAX6 in hESC-derived NPCs. Quantitative RT-PCR expression analysis for the NPC marker genes NESTIN, SOX2, MSI1, and PAX6, following chronic Pb exposure at days 0–19 (A) or days 11–19 (B) of the differentiation procedure (paradigms C and D in Fig. 1A). Histogram values represent means and error bars represent 95% interval confidences. Statistically significant differences (*p < 0.05; **p < 0.01; n = 5, one-way ANOVA) between Pb-exposed hESCs and controls. (C) Immunofluorescence staining for the NPC marker Nestin (green) and Msi1 (red) in NPCs derived from hESCs exposed to 0–1.9μM Pb between day 11 and day 19 of the differentiation protocol (paradigm D in Fig. 1A). (D) The percentage of Nestin-, Msi1-, and double-stained cells obtained from hESCs differentiated in 0.4–1.9μM Pb between day 11 and day 19 of the differentiation protocol did not differ from control percentages. Histogram values represent means ± SEM (n = 3, one-way ANOVA). Bar: 20 μm.