TABLE 2. A-Clustering Analysis of Pb-Induced Methylation Changes in Undifferentiated hESCs, Differentiating hESCs, and NPC0–19 (Paradigms A, B, and C in Fig. 1A). Table Gives the Summary of the Methylation Profiles for Each Experimental Group After Determining Differentially Methylated Clusters (DMCs) Using GEE (See the Materials and Methods section).
| Undifferentiated hESCs | Differentiating hESCs | NPC0–19 | |
|---|---|---|---|
| Number of clusters (before filtering) | 12785 | 11519 | 7167 |
| Significant clusters (after filtering) | 49 | 1982 | 73 |
| Total number of CpG sites mapped to DMCs | 163 | 6351 | 330 |
| Unique genes associated with DMCs | 29 | 1275 | 45 |
| DMCs with 2 CpG sites (%) | 59.2 | 53.6 | 41.1 |
| DMCs with 3 to 10 CpG sites (%) | 36.7 | 44.5 | 45.2 |
| DMCs with ≥ 10 CpG sites (%) | 4.1 | 1.9 | 13.7 |
| CpG sites associated with promoters (%) | 30.1 | 11.7 | 31.5 |
| CpG sites mapping to CpG resort (%) | 80.4 | 59.6 | 83.9 |