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. Author manuscript; available in PMC: 2014 May 16.
Published in final edited form as: Cell. 2013 Jul 18;154(2):297–310. doi: 10.1016/j.cell.2013.06.027

Figure 3. SET1C Facilitates p53- and p300-Dependent Transcription on Recombinant Chromatin through H3K4 Trimethylation.

Figure 3

(A) Scheme for recombinant chromatin-based transcription assays.

(B) Stimulation of p53-and p300-dependent transcription by SET1C.

(C) Effects of H3 and H4 mutations on SET1C- and p300-dependent transcription. Chromatin templates were reconstituted with either control (WT) or indicated mutant histones (detailed in Figure S3G).

(D) Stimulation of p53- and p300-dependent transcription by prior incorporation of semisynthetic H3Kc4me3 into the chromatin template. Factor additions as indicated in (B)–(D). Autoradiographs in (B), (C), and (D) were scanned and quantitated by Quantity One Program (Bio-Rad). See also Figures S1 and S3.