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. Author manuscript; available in PMC: 2014 May 16.
Published in final edited form as: Cell. 2013 Jul 18;154(2):297–310. doi: 10.1016/j.cell.2013.06.027

Figure 4. Direct Doxorubicin-Induced Interactions of p53 with SET1C in HCT116 Cells.

Figure 4

(A) Doxorubicin-induced interaction of p53 with endogenous SET1C. IPs (left) were monitored by immunoblot with indicated antibodies (right).

(B) SET1C-specific interaction with p53. IPs (indicated at the top) from doxorubicin-treated (16 hr) p53 WT and p53 null HCT116 cells were monitored by immunoblot with indicated antibodies (right).

(C) Direct binding of purified SET1C (monitored by antibodies indicated on right) to p53.

(D) Scheme for DSP-mediated crosslinking of p53 to SET1C.

(E) CFP1-mediated intracellular association of p53 with a reconstituted SET1C (details in the Extended Experimental Procedures). See also Figures S1 and S5.