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. Author manuscript; available in PMC: 2014 May 16.
Published in final edited form as: Cell. 2013 Jul 18;154(2):297–310. doi: 10.1016/j.cell.2013.06.027

Figure 5. p300 and SET1C Act Cooperatively to Regulate DNA-Damage-Induced Transcription of the p21/WAF1 Gene by p53.

Figure 5

(A) Effects of siRNA-based CFP1 and WDR82 knockdowns on global H3K4 methylation and doxorubicin-induced p53 target gene (p21/WAF1) expression in HCT116 cells. The asterisk indicates a cross-reactive band. In (A) and (E), siRNA pools are indicated at the top, and immunoblot antibodies on the right. The instability of SET1A in the absence of WDR82 was previously noted in yeast and human cells.

(B) Diagram of the p21/WAF1 gene and PCR amplicons used for ChIP analyses.

(C) Effects of siRNA-based p300, CFP1, and WDR82 depletions on doxorubicin-induced (16 hr) enrichment of H3K4me3 on 21/WAF1 in HCT116 p53+/+ cells. ChIP analysis at amplicons A through F as indicated in (B).

(D) Effects of siRNA-based CFP1 and WDR82 depletions on p21/WAF1 expression in doxorubicin-treated HCT116 p53+/+ cells. RNA levels were monitored by real-time RT-PCR. Errors bars in (C) and (D) denote SD from duplicate reactions by real-time PCR.

(E) Effects of p300 knockdown on global H3K18/27 acetylation and H3K4 methylation and on expression of p21/WAF1.