Fig. 5.

Effects of acute endotoxemia on C/EBP nuclear binding activity in adherent vascular lung neutrophils. Cells isolated from control (CTL) animals or 2 h after endotoxin (ETX) administration were treated with medium control (Med), IFN-γ (IFN), TPA, or TPA + IFN-γ (I + T) for 1 h. Nuclear extracts were analyzed for C/EBP binding activity by a gel retardation assay. Extracts prepared from IFN-γ treated cells were incubated on ice for 15 min with antibodies to C/EBP-β and/or C/EBP-δ (1 mg), or 40-fold excess of unlabeled cold competitor (Comp) prior to the labeled probe. One representative gel from three separate experiments is shown.