(A)Representative recordings from four/64 MEA electrodes recorded from control (11a, 18a) and C9orf72 expansion ALS-derived neurons (19f, RB8b) cultured for 14 days.
(B) Total action potential firing rate during one minute of recording from MEAs (11a, n=1; 18a, n=3; control mean 4,752 ± 2,786 spikes/minute; 19f, n=3; RB8b, n=3; ALS mean 20,022 ± 3,775 spikes/minute; p<0.05, t-test).
(C) Average of mean firing rate for control and C9orf72-derived neurons (11a, n=82; 18a, n=203; control mean 1.11 ± 0.06 Hz; 19f, n=407; RB9d, n=929; ALS mean 1.50 ± 0.04; p<10−5, t-test).