Table 1.

Gastrointestinal intraepithelial neoplasia (GIN) incidence in male and female INS-GAS mice at timepoints equivalent to 7 mpi with Helicobacter pylori

Microbial status	% Of mice with no dysplasia (score=0)		% Of mice with non-GIN dysplasia* (score 0.5–2.5)		% Of mice with GIN (high-grade dysplasia) (score=3)		% Of mice with invasive neoplasms † (score >3)
	M (%)	F (%)	M (%)	F (%)	M (%)	F (%)	M (%)	F (%)
GF (M=6, F=6)	17	17	83	83	0	0	0	0
rASF (M=10, F=7)	0	14	100	86	0	0	0	0
IF (M=10, F=5)	10	20	90	80	0	0	0	0
mHp (M=6, F=6)	0	66	100	34	0	0	0	0
rASFHp (M=13, F=9)	0	0	31	100	46‡	0	23‡	0
IFHp (M=15, F=9)	0	0	7	100	53‡	0	40‡	0

^*Non-GIN dysplasia defined as low to moderate grade dysplasia (score 0.5–2.5).

^†Invasive neoplasms defined as either intramucosal carcinomas (extending into the lamina propria or muscularis mucosae) or carcinomas extending into or beyond the submucosal margins.

^‡similar incidence of high-grade dysplasia in 46% and 53%, and similar incidence of invasive neoplasms in 23% and 40% of rASFHp and IFHp mice, respectively, were significantly higher compared with GF, rASF, IF and mHp-colonised male mice (all p<0.05).

F, female INS-GAS mice; GF, germfree; IF, intestinal flora (undefined SPF); M, male; mHp, monoassociated with H pylori; rASF, restricted ASF.