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. 2014 May 12;15:63. doi: 10.1186/1471-2202-15-63

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Copyright © 2014 Shang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantitation of necrotic cells under hydrostatic pressure and Nec-1 usage. Panel A: Normal control cells; Panel B: RGC-5 cell lines necrosis under elevate hydrostatic pressure; Panel C: RGC-5 cell lines were treated with necrostatin-1 (10 μmol/L) to block necroptosis for 24 hr in cell density of 0.6 × 10⁵/ml before elevate hydrostatic pressure and analysis of necroptotic cells. Cells were stained with Annexin FITC and PI, and analyzed by FACS using FL1 (Annexin) and FL3 (PI) channels. Panel D: The statistical analysis of RGC-5 necrosis, * vs # p < 0.05.