Fig. 1.

CLSM images of live/dead biofilms on resin. The top titles indicate the bonding agents, and the left titles indicate whether the resin was pre-coated with human salivary pellicles, and the culture medium type. Bacteria with integral membrane were stained green, and bacteria with compromised membrane were stained red. Bacteria of those two cohorts that were close to or on the top of each other produced orange or yellow colors. It should be noted that some red cells may have partially lost membrane function and may still be viable. Control disks were covered with primarily live bacteria. Adhesives containing DMADDM or NAg greatly inhibited the microcosm biofilm growth.