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. Author manuscript; available in PMC: 2014 May 16.
Published in final edited form as: Allergy. 2008 Jun 10;63(9):1136–1147. doi: 10.1111/j.1398-9995.2008.01677.x

Figure 3.

Figure 3

Effect of PPARγ ligands on histamine contents and release of bone marrow-derived mast cells. (A) Schematic protocol of bone marrow-derived progenitor cell culture. (B) Time-course alterations of histamine content of bone marrow-derived mast cells (BMMCs) treated with PPARγ ligand, rosiglitazone or pioglitazone. Data represent mean ± SEM from five to seven independent experiments. (C) Dose-dependent alteration of histamine content of BMMCs treated with PPARγ ligand, rosiglitazone. Data represent mean ± SEM from three to five independent experiments. (D) Effect of PPARγ-specific antagonist, GW 9662, on histamine contents. Data represent mean from three independent experiments. (E) Recovery of decreased histamine content in BMMCs by the treatment with PPARγ-specific antagonist in the presence of PPARγ agonist (rosiglitazone, 10 µM, Rosi). Data represent mean ± SEM from three to five independent experiments. (F) Effect of PPARγ on the decrease in histamine contents using bone marrow-progenitors prepared from heterozygous PPARγ-deficient mice. Open column represents the histamine content in the presence of PPARγ agonist (rosiglitazone, 10 µM). Data represent mean from 3 independent experiments. (G) Effect of PPARγ ligand (rosiglitazone, 10 µM) on DNP-BSA antigen-stimulated histamine release from BMMCs. Data represent mean ± SEM from five to seven independent experiments. **P < 0.01 vs vehicle control. (H) Effect of PPARγ ligand (rosiglitazone, 10 µM) on DNP-BSA antigen-stimulated histamine release from BMMCs. Percentage of histamine release was calculated as ‘net histamine release/total contents of histamine × 100’. Data represent mean ± SEM from five to seven independent experiments. **P < 0.01 vs vehicle control. (I) Effect of PPARγ ligand (rosiglitazone, 10 µM) on DNP-BSA antigen-stimulated TNF-α production from BMMCs. Data represent mean ± SEM from four to seven independent experiments. **P < 0.01 vs vehicle control. (J) Effect of PPARγ on TNF-α production using bone marrow-progenitors prepared from heterozygous PPARγ-deficient mice. Data represent mean from three independent experiments. *P < 0.05 vs wild type.