Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 12;15:280. doi: 10.1186/1471-2164-15-280

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Bocsanczy et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Experimental design flowchart. a. Conditions of bacterial populations and type of proteins extracted by comparisons. b. Example of experimental design for the comparison of two strains. Protein samples to be compared were stained with either Cy3 or Cy5 fluorescent dyes. Three biological reps of each sample to be compared were combined in several gels to provide statistical power for comparisons. The pool of gels is normalized in order to compare spots from different gels. Spots were identified and localized across gels and their abundance compared statistically. A list of differentially expressed spots was analyzed and a subset of the total number of spots of interest were excised and sequenced by MS analysis.