Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 23;15:301. doi: 10.1186/1471-2164-15-301

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Xie; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Validation of MKL1 and ELK4 recruitment to target gene promoters following zymosan treatment. (A) MKL1 ChIP assay was performed in BLRP-MKL1-BirA stable cells under basal conditions and after zymosan treatment for 1 h. Immunoprecipitated DNA was analyzed by Q-PCR using primers flanking the proximal promoter regions of Srf and Vcl. (B) Conventional ELK4 ChIP assay was performed in BLRP-MKL1-BirA stable cells under basal conditions and after zymosan treatment for 1 h. Rabbit IgG was used as a negative control. Immunoprecipitated DNA was analyzed by qPCR using primers flanking the proximal promoter regions of c-fos and Egr1.