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. Author manuscript; available in PMC: 2014 May 16.
Published in final edited form as: Cell Stem Cell. 2012 Aug 2;11(3):359–372. doi: 10.1016/j.stem.2012.05.024

Figure 2. Expression of leukemia CRGs is conserved in mature and primitive populations.

Figure 2

Leukemic animals were generated as described for Figure 1. Bone marrow was harvested from 4 independent groups of mice (n = 5 per group) and purified based on expression of BCR-ABL and NUP98-HOXA9 using GFP and YFP markers, respectively. Normal and leukemic cells were further purified based on expression of lineage markers and the stem cell antigen Sca-1. As shown in the left panel, three distinct populations were isolated: lineage positive cells (population A), lineage negative progenitors (population B), and LSC-enriched, Lin−/Sca+ (population C). RNA was isolated from each purified population and analyzed using a custom Taqman Low Density Array (TLDA) designed to interrogate the CRGs. The right panel shows a heatmap of relative expression of the CRGs in mature, progenitor, and LSC-enriched leukemia cells compared to their respective normal counterparts (Green = down-regulated, Red = up-regulated).