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. Author manuscript; available in PMC: 2014 May 16.
Published in final edited form as: Cell Stem Cell. 2012 Aug 2;11(3):359–372. doi: 10.1016/j.stem.2012.05.024

Figure 6. Expression and function of leukemia CRGs is conserved in human blast crisis leukemia specimens.

Figure 6

(A) Total RNA was isolated from CD34-enriched human or primitive murine leukemia cells and analyzed by qRT-PCR using custom Taqman Low Density Arrays (TLDA) designed to interrogate the CRGs. The relative expression in leukemia compared to normal counterparts was determined (n=8 human, n= 6 mouse). The 13 genes conserved between the two species and their relative expression is shown. (B) Normal or leukemic human specimens were treated overnight with increasing concentrations of Tyrophostin AG825 (AG825). Twenty-four hours later the cells were harvested and labeled with annexin V and DAPI for analysis of cell death using flow cytometry. (C–D) Viability of bulk and primitive (lin−) human cells treated with AG825 for twenty-four hours. (E) Normal or leukemic human cells were treated with AG825 overnight followed by methylcellulose culture to measure colony formation ability, CFU (* p<0.01), ** p< 0.01, *** p< 0.001). See also Figure S7 and Tables S2.