Figure 2. Screenshots of the Galaxy tool for analyzing pooled genetic sequencing screens using edgeR.

( A) From the main screen, the user selects the appropriate input files and analysis options. ( B) The results of an analysis are summarized in an HTML page that includes various diagnostic plots. ( C) Output also includes a table of ranked results at the hairpin/guide and gene-level (where appropriate) as well as barcode plots ( D) that highlight the ranks of hairpins/guides targeting a specific gene relative to all other hairpins/guides in the data set.