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. Author manuscript; available in PMC: 2014 Nov 27.
Published in final edited form as: Sci Transl Med. 2013 Nov 27;5(213):213ra167. doi: 10.1126/scitranslmed.3007049

Fig. 2. Nanoparticle assembly, characterization, and in vitro transepithelial transport.

Fig. 2

(A) Schematic of NP-Fc assembly. NPs consist of a biodegradable PLA core for drug encapsulation and a PEG surface coating for particle stability and to reduce phagocytic uptake. NPs were formed using the nanoprecipitation self-assembly method (25) and surface-modified with IgG Fc for FcRn targeting. (B) Dynamic light scattering measurements for non-targeted NPs and NP-Fc. Data are means ± SD (n=3). (C) IgG Fc ligand density on the NP surface with (Fc-SH) and without (Fc) thiol modification of the IgG Fc. Data are means ± SD (n=3). (D) In vitro transepithelial transport of non-targeted NPs, NP-Fc, and NP-Fc with an excess of human IgG Fc as a blocking agent for FcRn. Data are expressed as mean basolateral 3H disintegrations per minute (DPM) as a percentage of the initial amount of 3H (± SEM; n = 4 wells per group). *P < 0.05, two-tailed Student’s t-test.