Table 1.
Primer sequences used for multiplex amplification of the whole mtDNA.
| Amplicon | Position | Sequence (5′-3′) | Size (bp) | Strand |
|---|---|---|---|---|
| 1 | 550–569 | AACCAAACCCCAAAGACACC | 2452 | F |
| 1 | 3001–2982 | CTGATCCAACATCGAGGTCG | R | |
| 2 | 2777–2797 | GTCCTAAACTACCAAACCTGC | 2417 | F |
| 2 | 5193–5174 | GTGTTAGTCATGTTAGCTTG | R | |
| 3 | 5042–5061 | AGCAGTTCTACCGTACAACC | 2476 | F |
| 3 | 7517–7497 | TTTGAAAAAGTCATGGAGGCC | R | |
| 4 | 7316–7336 | GATTTGAGAAGCCTTCGCTTC | 2524 | F |
| 4 | 9839–9819 | GCCAATAATGACGTGAAGTCC | R | |
| 5 | 9592–9611 | TCCCACTCCTAAACACATCC | 2539 | F |
| 5 | 12 130–12 111 | AAACCCGGTAATGATGTCGG | R | |
| 6 | 11 711–11 727 | GCCCACGGGCTTACATC | 2868 | F |
| 6 | 14 578–14 559 | GATTGTTAGCGGTGTGGTCG | R | |
| 7 | 14 111–14 130 | TCTTCCCACTCATCCTAACC | 2590 | F |
| 7 | 131–112 | ACAGATACTGCGACATAGGG | R | |
| 8 | 15 572–15 591 | TTCGCCTACACAATTCTCCG | 1643 | F |
| 8 | 645–626 | TTTATGGGGTGATGTGAGCC | R |
For, forward strand; Rev, reverse strand; nt, nucleotide; bp, base pair.