Table 3.
Extension primers for the multiplex detection of 21 haplogroups defining SNVs in two panels.
| Peak # | Hg | Polymorphism | For/Rev | Tested nt | bp | Sequence (59-39) |
|---|---|---|---|---|---|---|
| Panel | HT1 | |||||
| 1 | Hv | m.11719A>G | 19 | TTCTCATAATCGCCCACGG | ||
| 2 | U1811 | m.1811A>G | 27 | ACCGCAAGGGAAAGATGAAAAATTATA | ||
| 3 | N | m.9540C>T | 20 | TTCTAGCCCCTACCCCCCAA | ||
| 4 | B4bd | m.15535C>T | 27 | CAATTATACCCTAGCCAACCCCTTAAA | ||
| 5 | M | m.10400C>T | 33 | CTATGAGTGACTACAAAAAGGATTAGACTGAAC | ||
| 6 | L0 | m.13276A>G | 34 | (T)6CTCCACTTCAAGTCAACTAGGACTCATA | ||
| 7 | L2 | m.7146A>G | R | T>C | 33 | AGAAAGTTAGATTTACGCCGATGAATATGATAG |
| 8 | M7/D4 | m.9824T>C, A | R | A>G, T | 35 | (T)11GAAAGTTGAGCCAATAATGACGTG |
| 9 | U | m.12308A>G | R | T>C | 39 | (T)12ACTTTTATTTGGAGTTGCACCAAAATT |
| 10 | B4c | m.15346G>A | R | C>T | 39 | (T)21GTTTGATCCCGTTTCGTG |
| Panel | HT2 | |||||
| 1 | A | m.1736A>G | 22 | CCTTAGCCAAACCATTTACCCA | ||
| 2 | L1 | m.13789T>C | 21 | TTCCAAACAACAATCCCCCTC | ||
| 3 | E/M9 | m.4491G>A | R | C>T | 24 | CTGCAAAGATGGTAGAGTAGATGA |
| 4 | B4a | m.5465T>C | R | A>G | 27 | (T)7ATAGGTAGGAGTAGCGTGGT |
| 5 | J/T | m.4216T>C | 27 | CCACTCACCCTAGCATTACTTATATGA | ||
| 6 | R | m.12705T>C | R | A>G | 33 | AGCGGTAACTAAGATTAGTATGGTAATTAGGAA |
| 7 | N1 | m.10238T>C | 33 | CCCTTTCTCCATAAAATTCTTCTTAGTAGCTAT | ||
| 8 | M8/R30/B5 | m.8584G>A | 40 | (T)19ACAATCCTAGGCCTACCCGCC | ||
| 9 | L3/M/N | m.3594T>C | 39 | (T)24ACCCAACCCCCTGGT | ||
| 10 | D | m.5178C>A | R | G>T | 39 | (T)11GATGGAATTAAGGGTGTTAGTCATGTTA |
| 11 | H | m.7028T>C | 51 | (T)30CGACACGTACTACGTTGTAGC |
Haplogroups correspond to multiplex panels HT1 and HT2 in Figure 7.