FIG 1.

Amphotericin B susceptibility of S. cerevisiae (BY4743) and C. albicans (SN95) strains deleted in FEN1 and SUR4. All strains are diploids and in deletants, both alleles of the indicated genes are deleted. CaFEN12 is an ortholog of ScSUR4. Serial 10-fold dilutions of cells were spotted on synthetic complete agar plates with the indicated concentrations of AmB and incubated at 30°C for 2 days before being photographed. Comparable results were obtained with Scfen1 and Scsur4 deletants in an S. cerevisiae haploid strain (BY4741) background and in Cafen1Δ/Δ, Cafen12Δ/Δ, and double-deletant strains independently constructed in C. albicans strain SN95.