FIG 2.

Diagnostic PCRs for CaFEN1 (A) and CaFEN12 (B) in parent strain (SN95) and in strains deleted in either or both of the genes. Upstream forward and downstream reverse primers, external to flanking regions of homology used for targeted gene deletion, were used for diagnostic PCR. For CaFEN1 diagnostic PCRs, CaFEN1-DG-S and CaFEN1-DG-R1 were used, and the expected sizes of amplified products from SN95, Cafen1Δ/Δ, and Cafen1Δ/Δ Cafen12Δ/Δ strains are 1,982 bp, 880 bp, and 880 bp, respectively. For CaFEN12 diagnostic PCRs, CaFEN12-DG-S and CaFEN12-DG-R1 were used, and the expected sizes of products from SN95, Cafen12Δ/Δ, and Cafen1Δ/Δ Cafen12Δ/Δ strains are 1,821 bp, 909 bp, and 909 bp, respectively.