TABLE 2.

Fluoroquinolone MICs of 83 isolates and amino acid changes in their DNA topoisomerase genes^a

No. of isolates	Amino acid substitution for:									MIC (μg/ml)
	ParC			ParE			GyrA
	D78	S79	D83	K426	D435	E474	G79	S81	E85	CIP	LVX	MOX
1	—	—	—	—	—	—	—	—	—	4	2	0.25
3	—	F	—	—	—	—	—	—	—	4–8	1–4	0.25–0.5
6	—	Y	—	—	—	—	—	—	—	4–8	1–2	0.12–8
3	—	—	N	—	—	—	—	—	—	4–8	1–4	0.25–0.5
1	N	—	—	—‡	—‡	—‡	—‡	—‡	—‡	8	2	0.5
1	—	—	—	—	—	K	—	—	—	8	2	1
1	N	—		—	—	—	—	F	—	32	8	4
1	—	F	—	—	—	—	A	—	—	32	16	8
1	—	F	—	—	—	—	—	A	—	64	32	8
31	—	F	—	—	—	—	—	F	—	32–64	16–32	2–8
1	—	F	—	—	—	—	—	Y	—	32	16	2
1	—‡	Y	—‡	—‡	—‡	—‡	—‡	F‡	—‡	32	16	4
5	—	Y	—	—	—	—	—	F	—	32–64	16–32	2–4
1	—	Y	—	—	—	—	—	V	—	32	16	4
2	—	Y	—	—	—	—	—	Y	—	32	16	4
2	—	F	—	—	—	—	—	—	K	32	16	4
2	—	—	Y	—	—	—	—	F	—	32	16	2–4
1	—	—	—	—	N	—	—	A	—	64	32	1
1	—	—	—	—	N	—	—	F	—	32	16	2
1	—	—	—	—	N	—	—‡	F‡	—‡	32	16	8
2	—	F	N	—	—	—	—	F	—	64	32	8
1	—	F	Y	—	—	—	—	Y	—	64	32	8
1	—	Y	—	—	—	—	—	F	K	64	32	1
1	—	F	—	—	—	—	—	Y	K	64	32	4
4	—	F	—	—	—	—	—	F	K	64	32	4–8
1	—	Y	—	—	—	—	—	F	Q	64	32	16
2	—	F	—	—	N	—	—	F	—	128	64	8
1	—	Y	—	—	N	—	—	F	—	128	64	4
1	—	Y	—	N	—	—	—	F	—	64	32	64
1	—	—	N	—	—	K	—	Y	—	32	16	2
1	—	F	Y	N	—	—	—	V		128	64	8
1	—	F	—	—	N	—	—	F	K	128	64	64

^aOnly changes involved in resistance are shown. —, no change; ‡, the residue is located in a recombinant gene. Additional amino acid changes, not involved in resistance, were ParC N91D (the isolate with mosaic parC gene), ParE I460V (28 isolates), and GyrA S114G (the three isolates with mosaic gyrA genes). CIP, ciprofloxacin; LVX, levofloxacin; MOX, moxifloxacin.