TABLE 2.
Drug resistance-associated mutations among MDR-TB isolates from China
| Drug (no. of resistant isolates) | Locus | Codon/nucleotide change(s) | Amino acid/nucleotide change(s) | No. (%) of isolates | Other mutation(s) |
|---|---|---|---|---|---|
| INH (128) | katG | NMa | NM | 34 (26.6) | |
| GGC-AGC | Gly299Serc | 1 (0.8) | |||
| AGC-CGC | Ser302Arg | 1 (0.8) | |||
| AGC-ACA | Ser315Thr | 1 (0.8) | |||
| AGC-ACC | Ser315Thr | 72 (56.3) | |||
| AGC-ACC | Ser315Thr | 4 (3.1) | T(−8)C of inhA | ||
| 1 (0.8) | T(−8)A of inhA | ||||
| 1 (0.8) | C(−15)T of inhA | ||||
| 1 (0.8) | C(−34)T of inhA | ||||
| 1 (0.8) | G(−6)A of oxyR-ahpC intergenic region | ||||
| 2 (1.6) | C(−10)T of oxyR-ahpC intergenic region | ||||
| AGC-AAC | Ser315Asn | 6 (4.7) | |||
| AGC-AAC/GCC-ACC | Ser315Asn/Ala379Thr | 1 (0.8) | |||
| CTG-CCG | Leu378Pro | 1 (0.8) | C(−30)T of oxyR-ahpC intergenic region | ||
| ACT-ATT | Thr380Ile | 1 (0.8) | C(−15)T of inhA and C(−12)T of oxyR-ahpC intergenic region | ||
| inhA | NM | NM | 110 (85.9) | ||
| T-A | T(−8)A | 1 (0.8) | |||
| 1 (0.8) | Ser315Thr of katG | ||||
| T-C | T(−8)C | 4 (3.1) | Ser315Thr of katG | ||
| C-T | C(−15)T | 7 (5.5) | |||
| 1 (0.8) | Ser315Thr of katG | ||||
| 1 (0.8) | G(−6)A of oxyR-ahpC intergenic region | ||||
| 1 (0.8) | C(−30)T of oxyR-ahpC intergenic region | ||||
| 1 (0.8) | Thr380Ile of katG and C(−12)T of oxyR-ahpC intergenic region | ||||
| C-T | C(−34)T | 1 (0.8) | Ser315Thr of katG | ||
| oxyR-ahpC intergenic region | NM | NM | 111 (86.7) | ||
| G-A | G(−6)A | 3 (2.3) | |||
| 1 (0.8) | Ser315Thr of katG | ||||
| 1 (0.8) | C(−15)T of inhA | ||||
| C-T | C(−10)T | 1 (0.8) | |||
| 2 (1.6) | Ser315Thr of katG | ||||
| C(−12)T | 1 (0.8) | ||||
| 1 (0.8) | Thr380Ile of katG and C(−15)T of inhA | ||||
| C(−30)T | 3 (2.3) | ||||
| 1 (0.8) | C(−15)T of inhA | ||||
| 1 (0.8) | Leu378Pro of katG | ||||
| C(−39)T | 2 (1.6) | ||||
| RIF (128) | rpoBb | NM | NM | 8 (6.3) | |
| GGC-GTC/TCG-TTC | Gly457Valc/Ser531Leu | 1 (0.8) | |||
| CTG-CGG/CAC-TAC | Leu459Arg/His526Tyr | 1 (0.8) | |||
| GAG-GGG/CAC-TAC | Asp472Glyc/His526Tyr | 1 (0.8) | |||
| TTC-CTC/CTG-CCG | Phe505Leu/Leu511Pro | 1 (0.8) | |||
| ACC-CCC/CTG-CCG | Thr508Pro/Leu511Pro | 1 (0.8) | |||
| CAG-CAC/CAC-TAC | Gln510His/His526Tyr | 1 (0.8) | |||
| CTG-CCG | Leu511Pro | 1 (0.8) | |||
| CTG-CCG/AGC-GGC | Leu511Pro/Ser512Gly | 1 (0.8) | |||
| CTG-CCG/GAC-GGC | Leu511Pro/Asp516Gly | 2 (1.6) | |||
| GAC-GGC | Asp516Gly | 2 (1.6) | |||
| GAC-GTC | Asp516Val | 3 (2.3) | |||
| GAC-TAC | Asp516Tyr | 2 (1.6) | |||
| GAC-TAC/CAC-GAC | Asp516Tyr/His526Asp | 1 (0.8) | |||
| GAC-GCC/CTG-CCG | Asp516Ala/Leu533Pro | 1 (0.8) | |||
| GAC-GGC/CTG-CCG | Asp516Gly/Leu533Pro | 2 (1.6) | |||
| TCG-ATG | Ser522Metc | 1 (0.8) | |||
| CAC-GAC | His526Asp | 12 (9.4) | |||
| CAC-AAC | His526Asn | 1 (0.8) | |||
| CAC-CGC | His526Arg | 3 (2.3) | |||
| CAC-TAC | His526Tyr | 7 (5.4) | |||
| CAC-TTC | His526Phe | 1 (0.8) | |||
| CAC-TAC/GAG-GGG | His526Tyr/Glu541Gly | 1 (0.8) | |||
| TCG-TTG | Ser531Leu | 67 (52.3) | |||
| TCG-TTG/ATC-ACC | Ser531Leu/Ile561Thr | 1 (0.8) | |||
| TCG-TTT | Ser531Phe | 1 (0.8) | |||
| CTG-CCG | Leu533Pro | 2 (1.6) | |||
| CTG-CCG/GAA-GCA | Leu533Pro/Glu562Ala | 1 (1.6) | |||
| ATC-ACC | Ile572Thrc | 1 (0.8) | |||
| CAP (10) | tlyA | NM | NM | 10 (100.0) | |
| rrs | NM | NM | 4 (40.0) | ||
| A-G | A(1401)G | 6 (60.0) | |||
| KAN (13) | eis | NM | NM | 9 (69.2) | |
| G-A | G(−10)A | 3 (23.1) | |||
| C-T | C(−14)T | 1 (7.7) | |||
| rrs | NM | NM | 6 (46.2) | ||
| A-G | A(1401)G | 7 (53.8) | |||
| CAP and KAN (7) | tlyA | NM | NM | 7 (100.0) | |
| eis | NM | NM | 7 (100.0) | ||
| rrs | NM | NM | 1 (14.3) | ||
| A-G | A(1401)G | 6 (85.7) | |||
| OFX (60) | gyrA | NM | NM | 7 (11.7) | |
| GCC-TCC/GAC-GGC | Ala74Ser/Asp94Gly | 1 (1.7) | |||
| GAC-GGC | Asp89Gly | 1 (1.7) | |||
| GCG-GTG | Ala90Val | 11 (18.3) | |||
| GCG-GTG/GAC-GCC | Ala90Val/Asp94Ala | 1 (1.7) | |||
| TCG-CCG | Ser91Pro | 4 (6.7) | |||
| GAC-AAC | Asp94Asn | 4 (6.7) | |||
| GAC-CAC | Asp94His | 2 (3.3) | |||
| GAC-GCC | Asp94Ala | 7 (11.7) | |||
| GAC-GCC | Asp94Ala | 1 (1.7) | Thr500Pro of gyrB | ||
| GAC-GGC | Asp94Gly | 15 (25.0) | |||
| GAC-GGC | Asp94Gly | 1 (1.7) | Ala504Val of gyrB | ||
| GAC-TAC | Asp94Tyr | 5 (8.3) | |||
| gyrB | NM | NM | 57 (94.9) | ||
| GAC-AAC | Asp461Asn | 1 (1.7) | |||
| ACC-CCC | Thr500Pro | 1 (1.7) | Asp94Ala of gyrA | ||
| GCG-GTG | Ala504Val | 1 (1.7) | Asp94Gly of gyrA |
a
NM, no mutation.
b
Amino acid numbers are based on homologous mutations in Escherichia coli.
c
The mutation was not previously reported.