TABLE 2.
MICs in MH broth and hemolysis of hybrid peptidesa
| Organism | MIC (μM) |
|||||||
|---|---|---|---|---|---|---|---|---|
| Parent peptide |
Hybrid peptide or mutant |
|||||||
| TB | TA | KL12 | LG21 | FG21 | KG20 | LG21R19A | LG21W15AF20A | |
| Gram-negative bacteria | ||||||||
| Escherichia coli (lab strain) | 100 | >200 | 50 | 2 | 2 | 4 | 100 | 100 |
| Pseudomonas aeruginosa (ATCC 27853) | 100 | >200 | 100 | 4 | 2 | 4 | 200 | 100 |
| Klebsiella pneumonia (ATCC 13883) | 100 | >200 | >200 | 2 | 4 | 10 | 200 | 200 |
| Salmonella enterica (ATCC 14028) | 200 | >200 | >200 | 2 | 5 | 10 | >200 | >200 |
| Gram-positive bacteria | ||||||||
| Bacillus subtilis (lab strain) | 25 | 25 | 50 | 2 | 1 | 4 | 50 | 100 |
| Staphylococcus aureus (ATCC 25923) | 25 | 25 | 50 | 2 | 0.5 | 4 | 50 | 100 |
| Streptococcus pyogenes (ATCC 19615) | 25 | 25 | 50 | 2 | 3 | 10 | 100 | 200 |
| Enterococcus faecalis (ATCC 29212) | 50 | 50 | 100 | 5 | 10 | 10 | 100 | >200 |
a
MICs were also determined for the parent peptides TA, TB, and KL12 peptides for comparison. The percent hemolysis values at 100 μM were as follows: LG21, 8.5%; FG21, 9.9%; KG20, 2.4%; LG21R19A, 25%; and LG21W15AF20A, 60%.