FIG 3.

Analysis of membrane fluidity by electron paramagnetic resonance. Parasites were incubated with or without the Galf derivatives for 2 days and then incubated with spin label 5-DSA. (A) Treatment with compounds 1 and 2 at their respective IC₅₀ and with compounds 3, 5, and 6 at >400 μM concentrations; lane C represents untreated control parasites. (B) Reversal of rigidifying effect of compound 1 after treatment with Tween 20 and similar effects observed after treatment with the rigidifying control UDCA followed by Tween 20. Data are expressed as means ±SEM and are representative of three independent experiments; each condition was tested in triplicate. Significance of results is expressed as follows: ***, P < 0.001; **, P < 0.01; *, P < 0.05.