FIG 4.

Binding of FQs to E. coli DNA gyrase (EcGyr). (A) Titration of 10 nM MFX and CFX with various concentrations of the enzyme. The change in the anisotropy was plotted against the enzyme concentrations. (B) Effect of DNase I treatment on binding of MFX and CFX (10 nM) to EcGyr (250 nM). Proteinase K and DNase I treatments were carried out as described in the legend for Fig. 3.