FIG 5.

FQ binding to DNA gyrase and its individual subunits. (A) Titration of CFX and MFX with increasing M. tuberculosis DNA gyrase (MtGyr) and its individual subunits (MtGyrA and MtGyrB). (B) Titration with E. coli DNA gyrase (EcGyr) and its individual subunits (EcGyrA and EcGyrB). The change in anisotropy was plotted against concentrations of the holoenzyme or individual subunits. CFX or MFX at 10 nM were used in these assays. MtGyrA, M. tuberculosis GyrA; MtGyrB, M. tuberculosis GyrB; EcGyrA, E. coli GyrA; EcGyrB, E. coli GyrB subunit.