FIG 6.
Coverage around codon 193 of the HIV-1 integrase region. Top two panels summarize the coverage of the 10 HIV-1NL4-3 sequences (top) obtained from the clones used to calculate the intrinsic error rate of the assay (gray lines) and 6 HIV-1NL4-3 sequences obtained from the positive controls in six regular sequencing runs (blue lines). The middle panel includes the coverage of 8 patient-derived HIV-1 sequences (clinical samples labeled 13-xxx). Gray bar indicates the homopolymeric region at and upstream of codon 193, i.e., 25 nucleotides in bold in the nucleotide alignment. Red vertical line depicts codon 193. The bottom panel shows the nucleotide sequences of the 8 patient-derived HIV-1 sequences.