TABLE 1.
Sensitivity of reverse transcription-PCR amplification of the C2V3 region of the HIV-1 env gene (480 nt)
| Viral load (copies/ml) | % positive samples by RT-PCR (no. of positive samples/total no. of samples tested)a |
|---|---|
| 1,001–5,000 | 95 (19/20) |
| 5,001–10,000 | 85 (17/20) |
| >10,000 | 95 (37/39) |
a
Reverse transcription-PCR amplification of patient-derived env fragments was performed with plasma samples (n = 79) from HIV-infected individuals with viral loads ranging from 1,000 to >10,000 copies of viral RNA/ml, as described in Materials and Methods.