TABLE 1.
Analysis of promoter transcription fusion constructs
| Strain | C23O activity (Uc/OD450)a |
|---|---|
| KJ(pSmeOxylE) | 18 ± 1.7 |
| KJΔRo(pSmeOxylE) | 89 ± 9.2 |
| KJ(pSmeRoxylE) | 20 ± 3.0 |
| KJΔRo(pSmeRoxylE) | 54 ± 4.5 |
| KJ(pPCMxylE) | 119 ± 17 |
| KJΔRo(pPCMxylE) | 115 ± 14 |
| KJ(pTolCxylE) | 5 ± 1 |
| KJΔRo(pTolCxylE) | 8 ± 1 |
a
One unit of catechol 2,3-dioxygenase (Uc) is defined as 1 nmol of catechol hydrolyzed per min. Results are expressed as the means ± standard deviations of three independent determinations. OD450, optical density at 450 nm.