FIG 2.
Genetic organization and EMSAs with BCAL2823 and BCAL2823CU4. (A) Genetic organization of RND-4 operon and BCAL2823. RND-4 transcriptional start site position is indicated by the smaller arrow. The mutations in BCAL2823 are marked with asterisks. (B) A 3.39 nM 6-FAM-labeled probe was used, with increasing amounts of BCAL2823 (indicated above each lane). The bound BCAL2823 is indicated by arrows. −, control. (C) A 3.39 nM 6-FAM-labeled probe was used, with increasing amounts of BCAL2823CU4 (indicated above each lane) or 35 pmol BCAL2823. − and + indicate absence or presence, respectively, of BCAL2823 protein.