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Replication fitness of multiple NNRTI-resistant HIV-1 variants in the presence of ETV and/or FTC. The relative prevalence of 12 viruses, each containing a single resistance substitution, as determined by longitudinal deep sequencing of viral RNA from culture supernatant from cells infected with pooled virus and exposed to no drug (A), 200 nM ETV (B), 200 nM ETV and 10 μM FTC (C), or 10 μM FTC (D). Data are from a single determination.
