TABLE 1.
Susceptibility to FTC, 3TC, tenofovir, RPV, and ETV of HIV-1 viruses containing substitutions at codon E138a
| Virus | FTC |
3TC |
Tenofovir |
RPV |
ETV |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| EC50 (μM) | Fold R (P value) | EC50 (μM) | Fold R (P value) | EC50 (μM) | Fold R (P value) | EC50 (nM) | Fold R (P value) | EC50 (nM) | Fold R (P value) | |
| WT | 1.2 ± 0.4 | 5.8 ± 2.5 | 16.1 ± 6.3 | 0.3 ± 0.1 | 1.3 ± 0.2 | |||||
| E138A mutant | 6.0 ± 2.3 | 4.7 (0.006) | 35.1 ± 10.0 | 6.0 (0.01) | 8.7 ± 2.4 | 0.5 (>0.05) | 1.5 ± 0.2 | 5.6 (0.03) | 2.9 ± 0.5 | 2.2 (0.05) |
| E138G mutant | 0.5 ± 0.1 | 0.4 (>0.05) | 0.7 ± 0.4 | 2.5 (0.05) | 3.2 ± 0.2 | 2.5 (0.05) | ||||
| E138K mutant | 2.5 ± 1.7 | 1.9 (>0.05) | 10.7 ± 6.7 | 1.8 (>0.05) | 6.6 ± 3.8 | 0.4 (0.05) | 0.8 ± 0.2 | 3.0 (0.04) | 2.8 ± 0.3 | 2.2 (0.05) |
| E138Q mutant | 0.4 ± 0.1 | 0.3 (>0.05) | 5.5 ± 2.4 | 0.3 (0.05) | 1.3 ± 0.1 | 4.8 (0.04) | 4.0 ± 0.1 | 3.1 (0.01) | ||
| E138R mutant | 1.3 ± 0.3 | 1.0 (>0.05) | 1.6 ± 0.4 | 6.0 (0.05) | 5.3 ± 1.6 | 4.1 (0.04) | ||||
a
EC50s are the concentrations of drug required to inhibit viral replication by 50% from 3 independent experiments that were log10 transformed and compared for statistically significant differences (P < 0.05) by using the two-sample Student paired t test. Data are reported as means ± standard deviations from 3 independent experiments. Fold R is the mean fold change in EC50 of mutant versus WT virus.