FIG 4.

GABAergic inhibitory transmission is reduced in daDREAM hippocampus. (A and B) Real-time qPCR analysis of indicated GABAR subunits (A) and GABAergic markers (B) in wild-type (wt) and transgenic (tg) hippocampus. Results are the means ± SEM from 8 to 12 mice in two independent experiments. *, P < 0.05, and ***, P < 0.001 (one-way ANOVA, Tukey's multiple comparison). (C) Western blot analysis of PSD95 and β3 GABA receptor proteins in wild-type and transgenic hippocampus. β-Actin content was used as loading control. Representative autoradiograms are shown. Densitometric quantification of the specific immunoreactive band versus loading control β-actin, from a total of 4 (PSD95) or 8 (GABARβ3) samples from each genotype, is shown. **, P = 0.0039 (two-tailed, unpaired t test). (D) Seizure severity after convulsant administration (PTZ, 45 mg/kg) in wild-type (wt, n = 7) and transgenic mice (tg, n = 8). **, P = 0.0083 (Mann-Whitney test).