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. 2014 Apr 11;6(5):590–603. doi: 10.1002/emmm.201403841

Figure 2.

Figure 2

Addition of CpG-B boosts OVA-specific B-cell responses.

A Fourteen days after s.c. immunisation with 100 μg of NP-OVA in IFA, dLN and sera were analysed to estimate NP-specific B cells and Ig levels, respectively. Representative example of FACS profiles: NP-specific B cells (left panel), among which plasma cells (PC: CD138+; middle panel) and GC-B cells (CD138−B220+GL-7+CD95+; right panel).

B Numbers of NP-specific GC-B cells and NP-specific PC in dLN after immunisation with IFA or IFA+CpG-B (n = 5/group; mean ± s.e.m.).

C Levels of NP-specific IgG in sera after s.c. immunisation with NP-OVA in IFA or IFA+CpG-B (n = 5/group, mean ± s.e.m.).

D 14 days after s.c. immunisation with 100 μg of OVA, numbers of OVA-specific GC-B cells and OVA-specific PC in dLN (n = 5/group; mean ± s.e.m.).

E, F Levels of OVA-specific IgG in sera of mice 14 days after s.c. immunisation with OVA in adjuvant or adjuvant+CpG-B (n = 5/condition, mean ± s.e.m.) (E) and over time after s.c. immunisation using IFA (F).

Data information: Data are representative of at least three independent experiments. ns, nonsignificant; *P < 0.05; **P ≤ 0.01; ***P ≤ 0.005; ****P ≤ 0.001.