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. 2014 Apr 11;6(5):590–603. doi: 10.1002/emmm.201403841

Figure 3.

Figure 3

Impact of CpG-B on Tfh-cell differentiation relies on CD11c+ cells.

A, BEight weeks after reconstitution, chimeric JHT (70%) + TLR9−/− (30%) → C57Bl/6 were s.c. immunised with 1W1K or OVA in IFA or IFA+CpG-B. Frequency of Tfh among 1W1K-specific CD4+ T cells in dLN 9 days after s.c. immunisation (n = 5/group, mean ± s.e.m.) (A) and levels of OVA-specific IgG, IgG1, IgG2b and IgG2c in the sera of mice 14 days after s.c. immunisation (n = 5/condition, mean ± s.e.m.) (B).

C Five days after i.p. injection of JHT or C57Bl/6 littermates with 40 μg of 1W1K in Alum or Alum+CpG-B, spleens were collected and analysed. Frequency of Tfh among 1W1K-specific CD4+ T cells 9 days after i.p. injection is shown as well as serum concentrations of IL-6 estimated by ELISA 6 h after the i.p. injection (n = 5/group mean ± s.e.m.).

D Eight weeks after reconstitution, chimeric CD11cDTR (70%) + (30%) C57Bl/6 or TLR9−/− → C57Bl/6 mice were treated every 2 days with DTx and s.c. immunised with 1W1K or OVA in IFA or IFA+CpG-B. Frequency of Tfh among 1W1K-specific CD4+ T cells in dLN 9 days after s.c. immunisation (n = 5/group, mean ± s.e.m.) and levels of OVA-specific IgG, in the sera of mice 14 days after s.c. immunisation (n = 5/group, mean ± s.e.m.).

Data information: Data are representative of at least three independent experiments. ns, nonsignificant; *P < 0.05; **P ≤ 0.01; ****P ≤ 0.001.