Figure 4.

Ag-presenting DC in the dLN are CD11b⁺ conventional and monocyte-derived DC.

A DC that captured and present Ag (CD11c⁺FITC⁺Y-Ae⁺) in dLN of mice 2 days after s.c. immunisation with Ea-FITC emulsified in IFA.

B Histograms of Y-Ae staining correspond to mice s.c. immunised either with OVA-FITC or Ea-FITC in IFA.

C Kinetics of Y-Ae⁺ FITC⁺ CD11c⁺ cells in the dLN after s.c. immunisation after IFA or IFA+CpG-B (IFA, open circle; IFA+CpG-B, solid circle) (n = 4/condition, mean ± s.e.m.).

D, E Two days after s.c. immunisation with Ea-FITC in IFA or IFA+CpG-B, Y-Ae⁺ FITC⁺CD11c⁺ cells from dLN were analysed for CD8, CD11b expression (D) and for CD64 (E).

F Kinetics of conventional DC (cDC, Y-Ae⁺ FITC⁺ CD11c⁺ CD11b⁺ CD64⁻) and monocyte-derived DC (moDC, Y-Ae⁺ FITC⁺ CD11c⁺ CD11b⁺ CD64⁺) in the dLN after s.c. immunisation with IFA or IFA+CpG-B (IFA, open circle; IFA+CpG-B, solid circle) (n = 3/group, mean ± s.e.m.).

Data information: Data are representative of at least five independent experiments.