Figure 7.

moDC drive the increase in Tfh-cell development due to CpG-B adjuvantation.

A C57Bl/6 animals were s.c. immunised with 1W1K or OVA in IFA or IFA+CpG-B and treated twice with liposomes containing either PBS or clodronate. Nine days after s.c. immunisation with 1W1K, monocyte depletion was assessed in the blood of animals (data not shown) and the frequency of Tfh among 1W1K-specific CD4⁺ T cells was estimated in the corresponding animals (n = 5/group, mean ± s.e.m.). Fourteen days after s.c. immunisation with OVA, total OVA-specific IgG were estimated by ELISA (n = 5/group, mean ± s.e.m.).

B–F Chimeric C57Bl/6 → C57Bl/6 (C57Bl/6) and CCR2^−/− → C57Bl/6 (CCR2^−/−) mice 8 weeks after reconstitution (B), CXC3CR1 and wild-type littermates (C), chimeric CD11c-DTR (70%) + C57Bl/6 (30%) → C57Bl/6 (C57Bl/6) and CD11cDTR (70%) + CX3CR1^−/− (30%) → C57Bl/6 (CX3CR1^−/−) mice treated three times with DTx (D), chimeric CCR2^−/− (70%) + C57Bl/6 (30%) → C57Bl/6 (C57Bl/6) and CCR2^−/− (70%) + MyD88^−/− (30%) → C57Bl/6 (MyD88^−/−) mice (E) and chimeric CCR2^−/− (70%) + C57Bl/6 (30%) → C57Bl/6 (C57Bl/6) and CCR2^−/− (70%) + I-Ab^−/− (30%) → C57Bl/6 (I-Ab^−/−) mice (F) were s.c. immunised with 1W1K in IFA or IFA+CpG-B. Nine days after s.c. immunisation, the frequency of Tfh among 1W1K-specific CD4⁺ T cells was estimated in the corresponding animals (n = 5/group, mean ± s.e.m.).

G C57Bl/6 animals were immunised s.c. in the ear with 1W1K in IFA or IFA+CpG-B. 1 h after immunisation, ear was resected and the frequency of Tfh among 1W1K-specific CD4⁺ T cells was estimated in the corresponding animals 9 days after (n = 5/group, mean ± s.e.m.).

Data information: Data are representative of at least three independent experiments. ns, nonsignificant; *P < 0.05; **P ≤ 0.01.