Figure 3. Incorporation of ALIX-h30-eGFP is dependent on the Gag late domains.

293T cells were transfected with either RΔ8.2 variants (WT or ΔYP) alone or with increasing amounts of the ALIX-h30-GFP expressing vector. 24 h post-transfection, cell lysates and VLPs were analyzed by western blot using the indicated antibodies.