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. 2014 May 16;9(5):e96950. doi: 10.1371/journal.pone.0096950

Figure 5. Incorporation of ALIX-h30-eGFP into forming HIV-1 and EIAV VLPs.

Figure 5

HeLa-C1 cells stably expressing ALIX-h30-eGFP were transfected with either HIV-1 Gag and Gag-mCherry plasmids (A and B) or EIAV Gag and Gag-mCherry plasmids (C and D). In all, ALIX arrives at or near the completion of Gag assembly. In HIV-1 case, we observed 44 VLP assembly events incorporating ALIX; 34 out of 44 events were similar to B in which no residual ALIX was detected after the initial transient recruitment, and 10 out of 44 events were similar to A in which some ALIX was left in the VLP. In EIAV case, we observed 33 EIAV VLP assembly events; retention of the ALIX is significantly higher in EIAV with 18 events that retained almost all of the ALIX as shown in D.