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. 2014 May 16;9(5):e97165. doi: 10.1371/journal.pone.0097165

Figure 1. Immunostaining for TLR2, PECAM-1, podoplanin, and VE-cadherin.

Figure 1

Mouse and human leukocyte cell line J774A.1 and HL60 were immunostained with both anti-PECAM-1 (CD31) and anti-TLR2, and nuclei were stained by DAPI. In the mouse heart section, lymphatic vessels (arrowheads) were immunostained by anti-podoplanin (PDPN) and a blood vessel (arrows) was stained by anti-VE-cadherin (VE-cad). In the human kidney section with type II diabetic nephropathy, there are glomerular endothelial cells immunostained with both anti-PECAM-1 (CD31) and anti-TLR2 (arrows), and cells that are only immunostained with anti-TLR2 (arrowheads). Bar: 20 µm.